Journal article
Protein Interactome Analysis of the Type IX Secretion System Identifies PorW as the Missing Link between the PorK/N Ring Complex and the Sov Translocon
DG Gorasia, IL Silva, CA Butler, M Chabalier, T Doan, E Cascales, PD Veith, EC Reynolds
Microbiology Spectrum | Published : 2022
Open access
Abstract
The type IX secretion system (T9SS) transports cargo proteins through the outer membrane of Bacteroidetes and attaches them to the cell surface for functions including pathogenesis, gliding motility, and degradation of carbon sources. The T9SS comprises at least 20 different proteins and includes several modules: the trans-envelope core module comprising the PorL/M motor and the PorK/N ring, the outer membrane Sov translocon, and the cell attachment complex. However, the spatial organization of these modules is unknown. We have characterized the protein interactome of the Sov translocon in Porphyromonas gingivalis and identified Sov-PorV-PorA as well as Sov-PorW-PorN-PorK to be novel network..
View full abstractRelated Projects (2)
Grants
Awarded by Aix-Marseille Université
Funding Acknowledgements
The work in E.C.R.'s laboratory was supported by the Australian National Health and Medical research Council grants ID 1193647 and ID 1123866, the Australian Research Council grant DP200100914, and the Australian Dental Research Foundation grant 3492018. The work on T9SS in E.C.R.'s laboratory was funded by the Centre National de la Recherche Scientifique, the Aix-Marseille Universite, and grants from the Agence Nationale de la Recherche (ANR-15-CE11-0019, ANR-20-CE11-0011) and from the Excellence Initiative of Aix-Marseille Universite, a French "Investissements d'Avenir" program (A*MIDEX AAP-ID-17-33). The PhD thesis of I.L.S. has been funded by the ANR grant.